Unknown

Dataset Information

0

MiRNA-1469 promotes lung cancer cells apoptosis through targeting STAT5a.


ABSTRACT: MicroRNAs play key roles in cell growth, differentiation, and apoptosis. In this study, we described the regulation and function of miR-1469 in apoptosis of lung cancer cells (A549 and NCI-H1650). Expression analysis verified that miR-1469 expression significantly increased in apoptotic cells. Overexpression of miR-1469 in lung cancer cells increased cell apoptosis induced by etoposide. Additionally, we identified that Stat5a is a downstream target of miR-1469, which can bind directly to the 3'-untranslated region of the Stat5a, subsequently reducing both the mRNA and protein levels of Stat5a. Finally, co-expression of miR-1469 and Stat5a in A549 and NCI-H1650 cells partially abrogated the effect of miR-1469 on cell apoptosis. Our results show that miR-1469 functions as an apoptosis enhancer to regulate lung cancer apoptosis through targeting Stat5a and may become a critical therapeutic target in lung cancer.

SUBMITTER: Xu C 

PROVIDER: S-EPMC4449445 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

MiRNA-1469 promotes lung cancer cells apoptosis through targeting STAT5a.

Xu Chengshan C   Zhang Ling L   Li Hengheng H   Liu Zhihua Z   Duan Lianning L   Lu Chengrong C  

American journal of cancer research 20150215 3


MicroRNAs play key roles in cell growth, differentiation, and apoptosis. In this study, we described the regulation and function of miR-1469 in apoptosis of lung cancer cells (A549 and NCI-H1650). Expression analysis verified that miR-1469 expression significantly increased in apoptotic cells. Overexpression of miR-1469 in lung cancer cells increased cell apoptosis induced by etoposide. Additionally, we identified that Stat5a is a downstream target of miR-1469, which can bind directly to the 3'-  ...[more]

Similar Datasets

| S-EPMC4275704 | biostudies-literature
| S-EPMC4823029 | biostudies-literature
| S-EPMC7185146 | biostudies-literature
| S-EPMC7858139 | biostudies-literature
| S-EPMC9291185 | biostudies-literature
| S-EPMC3494421 | biostudies-literature
| S-EPMC4139332 | biostudies-literature
| S-EPMC391065 | biostudies-literature
| S-EPMC5740072 | biostudies-literature
| S-EPMC8795737 | biostudies-literature