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High-throughput RNA profiling via up-front sample parallelization.

ABSTRACT: We describe a method called modular, early-tagged amplification (META) RNA profiling that can quantify a broad panel of microRNAs or mRNAs simultaneously across many samples and requires far less sequence depth than existing digital profiling technologies. The method assigns quantitative tags during reverse transcription to permit up-front sample pooling before competitive amplification and deep sequencing. This simple, scalable and inexpensive approach improves the practicality of large-scale gene expression studies.

SUBMITTER: Narayan A

PROVIDER: S-EPMC4451056 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

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High-throughput RNA profiling via up-front sample parallelization.

Narayan Azeet A Bommakanti Ananth A Patel Abhijit A AA

Nature methods 20150302 4

We describe a method called modular, early-tagged amplification (META) RNA profiling that can quantify a broad panel of microRNAs or mRNAs simultaneously across many samples and requires far less sequence depth than existing digital profiling technologies. The method assigns quantitative tags during reverse transcription to permit up-front sample pooling before competitive amplification and deep sequencing. This simple, scalable and inexpensive approach improves the practicality of large-scale g ...[more]

PMID: 25730493

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