Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.
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ABSTRACT: Background
Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices.Results
For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices.Conclusion
A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.
SUBMITTER: Gonzalez D
PROVIDER: S-EPMC4455038 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.
Gonzalez Daniel D Melloni Chiara C Poindexter Brenda B BB Yogev Ram R Atz Andrew M AM Sullivan Janice E JE Mendley Susan R SR Delmore Paula P Delinsky Amy A Zimmerman Kanecia K Lewandowski Andrew A Harper Barrie B Lewis Kenneth C KC Benjamin Daniel K DK Cohen-Wolkowiez Michael M
Bioanalysis 20150101 9
<h4>Background</h4>Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices.<h4>Results</h4>For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml ...[more]
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