Dataset Information
Effects of ambient coarse, fine, and ultrafine particles and their biological constituents on systemic biomarkers: a controlled human exposure study.
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ABSTRACT: Background
Ambient coarse, fine, and ultrafine particles have been associated with mortality and morbidity. Few studies have compared how various particle size fractions affect systemic biomarkers.Objectives
We examined changes of blood and urinary biomarkers following exposures to three particle sizes.Methods
Fifty healthy nonsmoking volunteers, mean age of 28 years, were exposed to coarse (2.5-10 ?m; mean, 213 ?g/m3) and fine (0.15-2.5 ?m; mean, 238 ?g/m3) concentrated ambient particles (CAPs), and filtered ambient and/or medical air. Twenty-five participants were exposed to ultrafine CAP (< 0.3 ?m; mean, 136 ?g/m3) and filtered medical air. Exposures lasted 130 min, separated by ? 2 weeks. Blood/urine samples were collected preexposure and 1 hr and 21 hr postexposure to determine blood interleukin-6 and C-reactive protein (inflammation), endothelin-1 and vascular endothelial growth factor (VEGF; vascular mediators), and malondialdehyde (lipid peroxidation); as well as urinary VEGF, 8-hydroxy-deoxy-guanosine (DNA oxidation), and malondialdehyde. Mixed-model regressions assessed pre- and postexposure differences.Results
One hour postexposure, for every 100-?g/m3 increase, coarse CAP was associated with increased blood VEGF (2.41 pg/mL; 95% CI: 0.41, 4.40) in models adjusted for O3, fine CAP with increased urinary malondialdehyde in single- (0.31 nmol/mg creatinine; 95% CI: 0.02, 0.60) and two-pollutant models, and ultrafine CAP with increased urinary 8-hydroxydeoxyguanosine in single- (0.69 ng/mg creatinine; 95% CI: 0.09, 1.29) and two-pollutant models, lasting < 21 hr. Endotoxin was significantly associated with biomarker changes similar to those found with CAPs.Conclusions
Ambient particles with various sizes/constituents may influence systemic biomarkers differently. Endotoxin in ambient particles may contribute to vascular mediator changes and oxidative stress.
SUBMITTER: Liu L
PROVIDER: S-EPMC4455587 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Publications
Effects of ambient coarse, fine, and ultrafine particles and their biological constituents on systemic biomarkers: a controlled human exposure study.
Environmental health perspectives 20150116 6
<h4>Background</h4>Ambient coarse, fine, and ultrafine particles have been associated with mortality and morbidity. Few studies have compared how various particle size fractions affect systemic biomarkers.<h4>Objectives</h4>We examined changes of blood and urinary biomarkers following exposures to three particle sizes.<h4>Methods</h4>Fifty healthy nonsmoking volunteers, mean age of 28 years, were exposed to coarse (2.5-10 μm; mean, 213 μg/m3) and fine (0.15-2.5 μm; mean, 238 μg/m3) concentrated ...[more]
