Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology.
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ABSTRACT:
SUBMITTER: Knight GT
PROVIDER: S-EPMC4456773 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
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Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology.
Knight G T GT Sha J J Ashton R S RS
Chemical communications (Cambridge, England) 20150301 25
We describe a modular culture platform that enables spatiotemporal control of the morphology of 2D neural tissues derived from human pluripotent stem cells (hPSCs) by simply adding clickable peptides to the media. It should be widely applicable for elucidating how spatiotemporal changes in morphology and substrate biochemistry regulate tissue morphogenesis. ...[more]
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