NxRepair: error correction in de novo sequence assembly using Nextera mate pairs.
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ABSTRACT:
SUBMITTER: Murphy RR
PROVIDER: S-EPMC4458127 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
NxRepair: error correction in de novo sequence assembly using Nextera mate pairs.
Murphy Rebecca R RR O'Connell Jared J Cox Anthony J AJ Schulz-Trieglaff Ole O
PeerJ 20150602
Scaffolding errors and incorrect repeat disambiguation during de novo assembly can result in large scale misassemblies in draft genomes. Nextera mate pair sequencing data provide additional information to resolve assembly ambiguities during scaffolding. Here, we introduce NxRepair, an open source toolkit for error correction in de novo assemblies that uses Nextera mate pair libraries to identify and correct large-scale errors. We show that NxRepair can identify and correct large scaffolding erro ...[more]
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