Unknown

Dataset Information

0

Chemically functionalized single-walled carbon nanotubes enhance the glutamate uptake characteristics of mouse cortical astrocytes.


ABSTRACT: Using a radioactive glutamate uptake assay and immunolabeling, we report that single-walled carbon nanotubes, chemically functionalized with polyethylene glycol (SWCNT-PEG), delivered as a colloidal solute, cause an increase in the uptake of extracellular glutamate by astrocytes and an increase in the immunoreactivity of the glutamate transporter GLAST on their cell surface, which is likely a consequence of an increase in the immunoreactivity of glial fibrillary acidic protein. Additional corollary is that astrocytes exposed to SWCNT-PEG became larger and stellate, morphological characteristics of maturation and heightened activity of these glial cells. These results imply that SWCNT-PEG could potentially be used as a viable candidate for neural prosthesis applications, perhaps to alleviate the death toll of neurons due to glutamate excitotoxicity, a pathological process observed in brain and spinal cord injuries.

SUBMITTER: Gottipati MK 

PROVIDER: S-EPMC4459931 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Chemically functionalized single-walled carbon nanotubes enhance the glutamate uptake characteristics of mouse cortical astrocytes.

Gottipati Manoj K MK   Bekyarova Elena E   Haddon Robert C RC   Parpura Vladimir V  

Amino acids 20150403 7


Using a radioactive glutamate uptake assay and immunolabeling, we report that single-walled carbon nanotubes, chemically functionalized with polyethylene glycol (SWCNT-PEG), delivered as a colloidal solute, cause an increase in the uptake of extracellular glutamate by astrocytes and an increase in the immunoreactivity of the glutamate transporter GLAST on their cell surface, which is likely a consequence of an increase in the immunoreactivity of glial fibrillary acidic protein. Additional coroll  ...[more]

Similar Datasets

| S-EPMC7408470 | biostudies-literature
| S-EPMC2886730 | biostudies-literature
| S-EPMC6519950 | biostudies-literature
| S-EPMC4091642 | biostudies-literature
| S-EPMC7399653 | biostudies-literature
| S-EPMC5453357 | biostudies-other
| S-EPMC3292351 | biostudies-literature
| S-EPMC9503782 | biostudies-literature
| S-EPMC11337164 | biostudies-literature
| S-EPMC3675050 | biostudies-literature