Ontology highlight
ABSTRACT:
SUBMITTER: Nandakumar D
PROVIDER: S-EPMC4460406 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Nandakumar Divya D Pandey Manjula M Patel Smita S SS
eLife 20150513
Leading strand DNA synthesis requires functional coupling between replicative helicase and DNA polymerase (DNAP) enzymes, but the structural and mechanistic basis of coupling is poorly understood. This study defines the precise positions of T7 helicase and T7 DNAP at the replication fork junction with single-base resolution to create a structural model that explains the mutual stimulation of activities. Our 2-aminopurine studies show that helicase and polymerase both participate in DNA melting, ...[more]