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Multiple crystal forms of N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus.


ABSTRACT: Native N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus (Pf-Dac) and its selenomethionine derivative (Se-Pf-Dac) were crystallized and analyzed in the presence and absence of cadmium ion. The four crystal structures fell into three different crystal-packing groups, with the cadmium-free Pf-Dac and Se-Pf-Dac belonging to the same space group, with homologous unit-cell parameters. The crystal structures in the presence of cadmium contained distorted octahedral cadmium complexes coordinated by three chlorides, two O atoms and an S or Se atom from the N-terminal methionine or selenomethionine, respectively. The N-terminal cadmium complex was involved in crystal contacts between symmetry-related molecules through hydrogen bonding to the N-termini. While all six N-termini of Se-Pf-Dac were involved in cadmium-complex formation, only two of the Pf-Dac N-termini participated in complex formation in the Cd-containing crystal, resulting in different crystal forms. These differences are discussed in light of the higher stability of the Cd-Se bond than the Cd-S bond. This work provides an example of the contribution of cadmium towards determining protein crystal quality and packing depending on the use of the native protein or the selenomethionine derivative.

SUBMITTER: Nakamura T 

PROVIDER: S-EPMC4461325 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Multiple crystal forms of N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus.

Nakamura Tsutomu T   Niiyama Mayumi M   Hashimoto Wakana W   Ida Kurumi K   Abe Manabu M   Morita Junji J   Uegaki Koichi K  

Acta crystallographica. Section F, Structural biology communications 20150520 Pt 6


Native N,N'-diacetylchitobiose deacetylase from Pyrococcus furiosus (Pf-Dac) and its selenomethionine derivative (Se-Pf-Dac) were crystallized and analyzed in the presence and absence of cadmium ion. The four crystal structures fell into three different crystal-packing groups, with the cadmium-free Pf-Dac and Se-Pf-Dac belonging to the same space group, with homologous unit-cell parameters. The crystal structures in the presence of cadmium contained distorted octahedral cadmium complexes coordin  ...[more]

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