Ontology highlight
ABSTRACT:
SUBMITTER: Yu C
PROVIDER: S-EPMC4461869 | biostudies-literature | 2015 Feb
REPOSITORIES: biostudies-literature
Yu Chen C Liu Yanxia Y Ma Tianhua T Liu Kai K Xu Shaohua S Zhang Yu Y Liu Honglei H La Russa Marie M Xie Min M Ding Sheng S Qi Lei S LS
Cell stem cell 20150201 2
The bacterial CRISPR-Cas9 system has emerged as an effective tool for sequence-specific gene knockout through non-homologous end joining (NHEJ), but it remains inefficient for precise editing of genome sequences. Here we develop a reporter-based screening approach for high-throughput identification of chemical compounds that can modulate precise genome editing through homology-directed repair (HDR). Using our screening method, we have identified small molecules that can enhance CRISPR-mediated H ...[more]