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A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements.

ABSTRACT: We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

SUBMITTER: Francois P

PROVIDER: S-EPMC446242 | biostudies-literature | 2004 Jul

REPOSITORIES: biostudies-literature

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A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements.

Francois Patrice P Renzi Gesuele G Pittet Didier D Bento Manuela M Lew Daniel D Harbarth Stephan S Vaudaux Pierre P Schrenzel Jacques J

Journal of clinical microbiology 20040701 7

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology ...[more]

PMID: 15243102

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Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing.

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Project description: Not available

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