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Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis.

ABSTRACT: Bipolaris papendorfii has been reported as a fungal plant pathogen that rarely causes opportunistic infection in humans. Secondary metabolites isolated from this fungus possess medicinal and anticancer properties. However, its genetic fundamental and basic biology are largely unknown. In this study, we report the first draft genome sequence of B. papendorfii UM 226 isolated from the skin scraping of a patient. The assembled 33.4 Mb genome encodes 11,015 putative coding DNA sequences, of which, 2.49% are predicted transposable elements. Multilocus phylogenetic and phylogenomic analyses showed B. papendorfii UM 226 clustering with Curvularia species, apart from other plant pathogenic Bipolaris species. Its genomic features suggest that it is a heterothallic fungus with a putative unique gene encoding the LysM-containing protein which might be involved in fungal virulence on host plants, as well as a wide array of enzymes involved in carbohydrate metabolism, degradation of polysaccharides and lignin in the plant cell wall, secondary metabolite biosynthesis (including dimethylallyl tryptophan synthase, non-ribosomal peptide synthetase, polyketide synthase), the terpenoid pathway and the caffeine metabolism. This first genomic characterization of B. papendorfii provides the basis for further studies on its biology, pathogenicity and medicinal potential.

SUBMITTER: Kuan CS

PROVIDER: S-EPMC4463846 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis.

Kuan Chee Sian CS Yew Su Mei SM Toh Yue Fen YF Chan Chai Ling CL Ngeow Yun Fong YF Lee Kok Wei KW Na Shiang Ling SL Yee Wai-Yan WY Hoh Chee-Choong CC Ng Kee Peng KP

DNA research : an international journal for rapid publication of reports on genes and genomes 20150427 3

Bipolaris papendorfii has been reported as a fungal plant pathogen that rarely causes opportunistic infection in humans. Secondary metabolites isolated from this fungus possess medicinal and anticancer properties. However, its genetic fundamental and basic biology are largely unknown. In this study, we report the first draft genome sequence of B. papendorfii UM 226 isolated from the skin scraping of a patient. The assembled 33.4 Mb genome encodes 11,015 putative coding DNA sequences, of which, 2 ...[more]

PMID: 25922537

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Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis.

Publications

Dissecting the fungal biology of Bipolaris papendorfii: from phylogenetic to comparative genomic analysis.

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