Ontology highlight
ABSTRACT:
SUBMITTER: Tsai A
PROVIDER: S-EPMC4465488 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Tsai Albert A Petrov Alexey A Marshall R Andrew RA Korlach Jonas J Uemura Sotaro S Puglisi Joseph D JD
Nature 20120701 7407
The initiation of translation establishes the reading frame for protein synthesis and is a key point of regulation. Initiation involves factor-driven assembly at a start codon of a messenger RNA of an elongation-competent 70S ribosomal particle (in bacteria) from separated 30S and 50S subunits and initiator transfer RNA. Here we establish in Escherichia coli, using direct single-molecule tracking, the timing of initiator tRNA, initiation factor 2 (IF2; encoded by infB) and 50S subunit joining du ...[more]