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Steady-state cross-correlations for live two-colour super-resolution localization data sets.

ABSTRACT: Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.

SUBMITTER: Stone MB

PROVIDER: S-EPMC4467025 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Steady-state cross-correlations for live two-colour super-resolution localization data sets.

Stone Matthew B MB Veatch Sarah L SL

Nature communications 20150612

Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during ...[more]

PMID: 26066572

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