Dataset Information

Multigene Phylogeography of Bactrocera caudata (Insecta: Tephritidae): Distinct Genetic Lineages in Northern and Southern Hemispheres.

ABSTRACT: Bactrocera caudata is a pest of pumpkin flower. Specimens of B. caudata from the northern hemisphere (mainland Asia) and southern hemisphere (Indonesia) were analysed using the partial DNA sequences of the nuclear 28S rRNA and internal transcribed spacer region 2 (ITS-2) genes, and the mitochondrial cytochrome c oxidase subunit I (COI), cytochrome c oxidase subunit II (COII) and 16S rRNA genes. The COI, COII, 16S rDNA and concatenated COI+COII+16S and COI+COII+16S+28S+ITS-2 nucleotide sequences revealed that B. caudata from the northern hemisphere (Peninsular Malaysia, East Malaysia, Thailand) was distinctly different from the southern hemisphere (Indonesia: Java, Bali and Lombok), without common haplotype between them. Phylogenetic analysis revealed two distinct clades (northern and southern hemispheres), indicating distinct genetic lineage. The uncorrected 'p' distance for the concatenated COI+COII+16S nucleotide sequences between the taxa from the northern and southern hemispheres ('p' = 4.46-4.94%) was several folds higher than the 'p' distance for the taxa in the northern hemisphere ('p' = 0.00-0.77%) and the southern hemisphere ('p' = 0.00%). This distinct difference was also reflected by concatenated COI+COII+16S+28S+ITS-2 nucleotide sequences with an uncorrected 'p' distance of 2.34-2.69% between the taxa of northern and southern hemispheres. In accordance with the type locality the Indonesian taxa belong to the nominal species. Thus the taxa from the northern hemisphere, if they were to constitute a cryptic species of the B. caudata species complex based on molecular data, need to be formally described as a new species. The Thailand and Malaysian B. caudata populations in the northern hemisphere showed distinct genetic structure and phylogeographic pattern.

SUBMITTER: Yong HS

PROVIDER: S-EPMC4474862 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Multigene Phylogeography of Bactrocera caudata (Insecta: Tephritidae): Distinct Genetic Lineages in Northern and Southern Hemispheres.

Yong Hoi-Sen HS Lim Phaik-Eem PE Tan Ji J Song Sze-Looi SL Suana I Wayan IW Eamsobhana Praphathip P

PloS one 20150619 6

Bactrocera caudata is a pest of pumpkin flower. Specimens of B. caudata from the northern hemisphere (mainland Asia) and southern hemisphere (Indonesia) were analysed using the partial DNA sequences of the nuclear 28S rRNA and internal transcribed spacer region 2 (ITS-2) genes, and the mitochondrial cytochrome c oxidase subunit I (COI), cytochrome c oxidase subunit II (COII) and 16S rRNA genes. The COI, COII, 16S rDNA and concatenated COI+COII+16S and COI+COII+16S+28S+ITS-2 nucleotide sequences ...[more]

PMID: 26090853

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Project description:For sustaining ecosystem functions and services, environmental conservation strategies increasingly target to maintain the multiple facets of biodiversity, such as functional diversity (FD) and phylogenetic diversity (PD), not just taxonomic diversity (TD). However, spatial mismatches among these components of biodiversity can impose challenges for conservation decisions. Hence, understanding the drivers of biodiversity is critical. Here, we investigated the global distribution patterns of TD, FD, and PD of breeding Anatidae. Using null models, we clarified the relative importance of mechanisms that influence Anatidae community. We also developed random forest models to evaluate the effects of environmental variables on the Anatidae TD, FD, and PD. Our results showed that geographical variation in Anatidae diversity is hemispheric rather than latitudinal. In the species-rich Northern Hemisphere (NH), the three diversity indices decreased with latitude within the tropical zone of the NH, but increased in the temperate zone reaching a peak at 44.5-70.0°N, where functional and phylogenetic clustering was a predominant feature. In the Southern Hemisphere (SH), Anatidae diversity increased poleward and a tendency to overdispersion was common. In NH, productivity seasonality and temperature in the coldest quarter were the most important variables. Productivity seasonality was also the most influential predictor of SH Anatidae diversity, along with peak productivity. These findings suggested that seasonality and productivity, both consistent with the energy-diversity hypothesis, interact with the varying histories to shape the contrasting hemispheric patterns of Anatidae diversity. Phylogenetic diversity (PD) and FD underdispersion, widespread across the species-rich, seasonally productive mid-to-high latitudes of the NH, reflects a rapid evolutionary radiation and resorting associated with Pleistocene cycles of glaciation. The SH continents (and southern Asia) are characterized by a widespread tendency toward PD and FD overdispersion, with their generally species-poor communities comprising proportionately more older lineages in thermally more stable but less predictably productive environments.

Project description:Mating compatibility among recently colonized (wildish) populations of Bactrocera dorsalis (Hendel) from different geographic origins in Thailand was assessed through inter-regional mating tests. Outdoor octagonal nylon screen field cages containing single potted mango trees (Mangifera indica L.) were used. Sexual compatibility was determined using the index of sexual isolation (ISI), the male relative performance index (MRPI), and the female relative performance index (FRPI). The ISI values indicated that the northern population of Bactrocera dorsalis from Chiang Mai province was sexually compatible with the southern population of Bactrocera dorsalis (previously Bactrocera papayae) from Nakhon Si Thammarat province. The MRPI values showed that the northern males had a slightly higher tendency to mate than southern males, while the FRPI data reflected that females of both origins participated equally in matings. In all combinations there were no differences between homotypic and heterotypic couples in mating latency. Southern males tended to mate first with southern females, followed by northern males mating with northern females, while the latest matings involved heterotypic couples, in particular northern males mating with southern females. Overall, more couples were collected from higher parts of the field cage and the upper tree canopy, while there were no differences between the origins of flies in terms of elevation of couples within the cage. Laboratory assessments of fecundity showed no differences in the average number of eggs resulting from inter-regional crosses. Development of immature stages was also equal in the two hybrid crosses, with no differences found in the number of pupae produced, percentage pupal recovery, and percent adult emergence. The practical implication of this study is that colony of Bactrocera dorsalis derived from any northern or southern region of Thailand can potentially be used in sterile insect technique programs against this pest.

Dataset Information

Multigene Phylogeography of Bactrocera caudata (Insecta: Tephritidae): Distinct Genetic Lineages in Northern and Southern Hemispheres.

Publications

Multigene Phylogeography of Bactrocera caudata (Insecta: Tephritidae): Distinct Genetic Lineages in Northern and Southern Hemispheres.

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