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Osteopontin mediates an MZF1-TGF-?1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.

ABSTRACT: Interactions between tumor cells and cancer-associated fibroblasts (CAFs) in the tumor microenvironment significantly influence cancer growth and metastasis. Transforming growth factor-? (TGF-?) is known to be a critical mediator of the CAF phenotype, and osteopontin (OPN) expression in tumors is associated with more aggressive phenotypes and poor patient outcomes. The potential link between these two pathways has not been previously addressed. Utilizing in vitro studies using human mesenchymal stem cells (MSCs) and MDA-MB231 (OPN+) and MCF7 (OPN-) human breast cancer cell lines, we demonstrate that OPN induces integrin-dependent MSC expression of TGF-?1 to mediate adoption of the CAF phenotype. This OPN-TGF-?1 pathway requires the transcription factor, myeloid zinc finger 1 (MZF1). In vivo studies with xenotransplant models in NOD-scid mice showed that OPN expression increases cancer growth and metastasis by mediating MSC-to-CAF transformation in a process that is MZF1 and TGF-?1 dependent. We conclude that tumor-derived OPN engenders MSC-to-CAF transformation in the microenvironment to promote tumor growth and metastasis via the OPN-MZF1-TGF-?1 pathway.

SUBMITTER: Weber CE

PROVIDER: S-EPMC4476970 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Osteopontin mediates an MZF1-TGF-β1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.

Weber C E CE Kothari A N AN Wai P Y PY Li N Y NY Driver J J Zapf M A C MA Franzen C A CA Gupta G N GN Osipo C C Zlobin A A Syn W K WK Zhang J J Kuo P C PC Mi Z Z

Oncogene 20141222 37

Interactions between tumor cells and cancer-associated fibroblasts (CAFs) in the tumor microenvironment significantly influence cancer growth and metastasis. Transforming growth factor-β (TGF-β) is known to be a critical mediator of the CAF phenotype, and osteopontin (OPN) expression in tumors is associated with more aggressive phenotypes and poor patient outcomes. The potential link between these two pathways has not been previously addressed. Utilizing in vitro studies using human mesenchymal ...[more]

PMID: 25531323

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Dataset Information

Osteopontin mediates an MZF1-TGF-?1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.

Publications

Osteopontin mediates an MZF1-TGF-β1-dependent transformation of mesenchymal stem cells into cancer-associated fibroblasts in breast cancer.

Similar Datasets

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