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Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

ABSTRACT: There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People's Republic of China.The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments.DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China.

SUBMITTER: Feng Y

PROVIDER: S-EPMC4477421 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

Feng Yun Y Fu Shi-Hong SH Yang Wei-Hong WH Zhang Yu-Zhen YZ He Biao B Tu Chang-Chun CC Liang Guo-Dong GD Zhang Hai-Lin HL

BMC research notes 20150623

<h4>Background</h4>There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People's Republic of China.<h4>Results</h4>The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An ...[more]

PMID: 26100251

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Dataset Information

Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

Publications

Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China.

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