Unknown

Dataset Information

0

Induction of antitumor immunity in vivo following delivery of a novel HPV-16 DNA vaccine encoding an E6/E7 fusion antigen.


ABSTRACT: Human papillomavirus type 16 (HPV-16) infection is associated with a majority of cervical cancers and a significant proportion of head and neck cancers. Here, we describe a novel-engineered DNA vaccine that encodes a HPV-16 consensus E6/E7 fusion gene (pConE6E7) with the goal of increasing its antitumor cellular immunity. Compared to an early stage HPV-16 E7 DNA vaccine (pE7), this construct was up to five times more potent in driving E7-specific cellular immune responses. Prophylactic administration of this vaccine resulted in 100% protection against HPV E6 and E7-expressing tumors. Therapeutic studies indicated that vaccination with pConE6E7 prevented or delayed the growth of tumors. Moreover, immunization with pConE6E7 could also partially overcome immune tolerance in E6/E7 transgenic mice. Such DNA immunogens are interesting candidates for further study to investigate mechanisms of tumor immune rejection in vivo.

SUBMITTER: Yan J 

PROVIDER: S-EPMC4477831 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Induction of antitumor immunity in vivo following delivery of a novel HPV-16 DNA vaccine encoding an E6/E7 fusion antigen.

Yan Jian J   Reichenbach Dawn K DK   Corbitt Natasha N   Hokey David A DA   Ramanathan Mathura P MP   McKinney Kibwei A KA   Weiner David B DB   Sewell Duane D  

Vaccine 20081118 3


Human papillomavirus type 16 (HPV-16) infection is associated with a majority of cervical cancers and a significant proportion of head and neck cancers. Here, we describe a novel-engineered DNA vaccine that encodes a HPV-16 consensus E6/E7 fusion gene (pConE6E7) with the goal of increasing its antitumor cellular immunity. Compared to an early stage HPV-16 E7 DNA vaccine (pE7), this construct was up to five times more potent in driving E7-specific cellular immune responses. Prophylactic administr  ...[more]

Similar Datasets

| S-EPMC3571619 | biostudies-literature
| S-EPMC7917608 | biostudies-literature
| S-EPMC18715 | biostudies-literature
| S-EPMC7606012 | biostudies-literature
| S-EPMC10209332 | biostudies-literature
| S-EPMC7825221 | biostudies-literature
2014-06-26 | GSE58841 | GEO
| S-EPMC3521877 | biostudies-literature
| S-EPMC8402821 | biostudies-literature
| S-EPMC9583550 | biostudies-literature