Ontology highlight
ABSTRACT:
SUBMITTER: Zhu J
PROVIDER: S-EPMC4478535 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Zhu Jin J Heinecke Dominic D Mulla Wahid A WA Bradford William D WD Rubinstein Boris B Box Andrew A Haug Jeffrey S JS Li Rong R
G3 (Bethesda, Md.) 20150330 6
Errors in mitosis are a primary cause of chromosome instability (CIN), generating aneuploid progeny cells. Whereas a variety of factors can influence CIN, under most conditions mitotic errors are rare events that have been difficult to measure accurately. Here we report a green fluorescent protein-based quantitative chromosome transmission fidelity (qCTF) assay in budding yeast that allows sensitive and quantitative detection of CIN and can be easily adapted to high-throughput analysis. Using th ...[more]