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Chemical lysis of cyanobacteria.

ABSTRACT: We have developed a mixture of enzymes and chemicals that completely lyse cyanobacteria. Since the treatment involves only readily-available chemicals and simple proteins that degrade the components of the cyanobacterial cell wall, it can easily be used in high-throughput applications requiring lysis for subsequent intracellular measurements. Our lysis technique consistently enables complete lysis of several different cyanobacterial strains, and we demonstrated that DNA, mRNA, and proteins are preserved in the lysates. Chemical lysis can be superior to existing techniques because of its convenience, reliability, and amenability to a variety of downstream applications.

SUBMITTER: Mehta KK

PROVIDER: S-EPMC4478636 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Chemical lysis of cyanobacteria.

Mehta Kunal K KK Evitt Niklaus H NH Swartz James R JR

Journal of biological engineering 20150605

We have developed a mixture of enzymes and chemicals that completely lyse cyanobacteria. Since the treatment involves only readily-available chemicals and simple proteins that degrade the components of the cyanobacterial cell wall, it can easily be used in high-throughput applications requiring lysis for subsequent intracellular measurements. Our lysis technique consistently enables complete lysis of several different cyanobacterial strains, and we demonstrated that DNA, mRNA, and proteins are p ...[more]

PMID: 26110017

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