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Comparative analysis of colorimetric staining in skin using open-source software.

ABSTRACT: Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.

SUBMITTER: Billings PC

PROVIDER: S-EPMC4479192 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Comparative analysis of colorimetric staining in skin using open-source software.

Billings Paul C PC Sanzari Jenine K JK Kennedy Ann R AR Cengel Keith A KA Seykora John T JT

Experimental dermatology 20150201 2

Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radi ...[more]

PMID: 25393687

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