Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe.
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ABSTRACT:
SUBMITTER: Asakawa H
PROVIDER: S-EPMC4483485 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
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Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe.
Asakawa Haruhiko H Mori Chie C Ohtsuki Chizuru C Iwamoto Masaaki M Hiraoka Yasushi Y Haraguchi Tokuko T
FEBS open bio 20150612
Essential nucleoporins Nup98 and Nup96 are coded by a single open reading frame, and produced by autopeptidase cleavage. The autocleavage site of Nup98-Nup96 is highly conserved in a wide range of organisms. To understand the importance of autocleavage, we examined a mutant that produces the Nup98-Nup96 joint molecule as a sole protein product of the nup189 (+) gene in the fission yeast Schizosaccharomyces pombe. Cells expressing only the joint molecule were found to be viable. This result indic ...[more]
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