Ontology highlight
ABSTRACT:
SUBMITTER: Karunatilaka KS
PROVIDER: S-EPMC4484588 | biostudies-literature | 2010 Oct
REPOSITORIES: biostudies-literature
Karunatilaka Krishanthi S KS Solem Amanda A Pyle Anna Marie AM Rueda David D
Nature 20101013 7318
DEAD-box helicases are conserved enzymes involved in nearly all aspects of RNA metabolism, but their mechanisms of action remain unclear. Here, we investigated the mechanism of the DEAD-box protein Mss116 on its natural substrate, the group II intron ai5γ. Group II introns are structurally complex catalytic RNAs considered evolutionarily related to the eukaryotic spliceosome, and an interesting paradigm for large RNA folding. We used single-molecule fluorescence to monitor the effect of Mss116 o ...[more]