Ontology highlight
ABSTRACT:
SUBMITTER: Olszko ME
PROVIDER: S-EPMC4490015 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Olszko M E ME Adair J E JE Linde I I Rae D T DT Trobridge P P Hocum J D JD Rawlings D J DJ Kiem H-P HP Trobridge G D GD
Gene therapy 20150319 7
Foamy virus (FV) vectors are promising for hematopoietic stem cell (HSC) gene therapy but preclinical data on the clonal composition of FV vector-transduced human repopulating cells is needed. Human CD34(+) human cord blood cells were transduced with an FV vector encoding a methylguanine methyltransferase (MGMT)P140K transgene, transplanted into immunodeficient NOD/SCID IL2Rγ(null) mice, and selected in vivo for gene-modified cells. The retroviral insertion site profile of repopulating clones wa ...[more]