LC-MS/MS-based serum proteomics for identification of candidate biomarkers for hepatocellular carcinoma.

ABSTRACT: Associating changes in protein levels with the onset of cancer has been widely investigated to identify clinically relevant diagnostic biomarkers. In the present study, we analyzed sera from 205 patients recruited in the United States and Egypt for biomarker discovery using label-free proteomic analysis by LC-MS/MS. We performed untargeted proteomic analysis of sera to identify candidate proteins with statistically significant differences between hepatocellular carcinoma (HCC) and patients with liver cirrhosis. We further evaluated the significance of 101 proteins in sera from the same 205 patients through targeted quantitation by MRM on a triple quadrupole mass spectrometer. This led to the identification of 21 candidate protein biomarkers that were significantly altered in both the Unite

SUBMITTER: Tsai TH 

PROVIDER: S-EPMC4490019 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature