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Expanded cytotoxic T-cell lymphocytes target the latent HIV reservoir.

ABSTRACT: Enhanced human immunodeficiency virus (HIV)-specific immunity may be required for HIV eradication. Administration of autologous, ex vivo expanded, virus-specific, cytotoxic T-lymphocytes derived from HIV-infected patients on suppressive antiretroviral therapy (HXTCs) are a powerful tool for proof-of-concept studies. Broadly specific, polyclonal HXTCs resulting from ex vivo expansion demonstrated improved control of autologous reservoir virus compared to bulk CD8(+) T cells in viral inhibition assays. Furthermore, patient-derived HXTCs were able to clear latently infected autologous resting CD4(+) T cells following exposure to the latency-reversing agent, vorinostat. HXTCs will be ideal reagents to administer with precise control in future in vivo studies in combination with latency-reversing agents.

SUBMITTER: Sung JA

PROVIDER: S-EPMC4490234 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Expanded cytotoxic T-cell lymphocytes target the latent HIV reservoir.

Sung Julia A JA Lam Sharon S Garrido Carolina C Archin Nancie N Rooney Cliona M CM Bollard Catherine M CM Margolis David M DM

The Journal of infectious diseases 20150113 2

Enhanced human immunodeficiency virus (HIV)-specific immunity may be required for HIV eradication. Administration of autologous, ex vivo expanded, virus-specific, cytotoxic T-lymphocytes derived from HIV-infected patients on suppressive antiretroviral therapy (HXTCs) are a powerful tool for proof-of-concept studies. Broadly specific, polyclonal HXTCs resulting from ex vivo expansion demonstrated improved control of autologous reservoir virus compared to bulk CD8(+) T cells in viral inhibition as ...[more]

PMID: 25589335

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