A proteomic analysis on human sperm tail: comparison between normozoospermia and asthenozoospermia.

ABSTRACT: Asthenozoospermia is a common cause of human male infertility characterized by reduced sperm motility. The molecular mechanism that impairs sperm motility is not fully understood. This study proposed to identify novel biomarkers by focusing on sperm tail proteomic analysis of asthenozoospermic patients.Sperm were isolated from normozoospermic and asthenozoospermic semen samples. Tail fractions were obtained by sonication followed by Percoll gradient. The proteins were extracted by solubilization and subjected to two-dimensional gel electrophoresis (2-DE); then, the spots were analyzed using Image Master 2D Platinum software. The significantly increased/decreased amounts of proteins in the two groups were exploited by matrix-assisted laser desorption-ionization time-of-flight/time-of-flight (MALDI-TOF-TOF) mass spectrometry.Three hundred ninety protein spots were detected in both groups. Twenty-one protein spots that had significantly altered amounts (p?

SUBMITTER: Hashemitabar M 

PROVIDER: S-EPMC4491089 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature