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Lucilia cuprina genome unlocks parasitic fly biology to underpin future interventions.

ABSTRACT: Lucilia cuprina is a parasitic fly of major economic importance worldwide. Larvae of this fly invade their animal host, feed on tissues and excretions and progressively cause severe skin disease (myiasis). Here we report the sequence and annotation of the 458-megabase draft genome of Lucilia cuprina. Analyses of this genome and the 14,544 predicted protein-encoding genes provide unique insights into the fly's molecular biology, interactions with the host animal and insecticide resistance. These insights have broad implications for designing new methods for the prevention and control of myiasis.

SUBMITTER: Anstead CA

PROVIDER: S-EPMC4491171 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Lucilia cuprina genome unlocks parasitic fly biology to underpin future interventions.

Anstead Clare A CA Korhonen Pasi K PK Young Neil D ND Hall Ross S RS Jex Aaron R AR Murali Shwetha C SC Hughes Daniel S T DS Lee Siu F SF Perry Trent T Stroehlein Andreas J AJ Ansell Brendan R E BR Breugelmans Bert B Hofmann Andreas A Qu Jiaxin J Dugan Shannon S Lee Sandra L SL Chao Hsu H Dinh Huyen H Han Yi Y Doddapaneni Harsha V HV Worley Kim C KC Muzny Donna M DM Ioannidis Panagiotis P Waterhouse Robert M RM Zdobnov Evgeny M EM James Peter J PJ Bagnall Neil H NH Kotze Andrew C AC Gibbs Richard A RA Richards Stephen S Batterham Philip P Gasser Robin B RB

Nature communications 20150625

Lucilia cuprina is a parasitic fly of major economic importance worldwide. Larvae of this fly invade their animal host, feed on tissues and excretions and progressively cause severe skin disease (myiasis). Here we report the sequence and annotation of the 458-megabase draft genome of Lucilia cuprina. Analyses of this genome and the 14,544 predicted protein-encoding genes provide unique insights into the fly's molecular biology, interactions with the host animal and insecticide resistance. These ...[more]

PMID: 26108605

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Project description:BackgroundThe sterile insect technique (SIT) has been successfully used in many pest management programs worldwide. Some SIT programs release both sexes due to the lack of genetic sexing strains or efficient sex separation methods but sterile females are ineffective control agents. Transgenic sexing strains (TSS) using the tetracycline-off control system have been developed in a variety of insect pests, from which females die by either of two commonly used lethal effectors: overexpression of the transcription factor tetracycline transactivator (tTA) or ectopic expression of a proapoptotic gene, such as head involution defective (hid). The lethality from tTA overexpression is thought to be due to "transcriptional squelching", while hid causes lethality by induction of apoptosis. This study aims to create and characterize a TSS of Lucilia cuprina, which is a major pest of sheep, by combining both lethal effectors in a single transgenic strain.ResultsHere a stable TSS of L. cuprina (DH6) that carries two lethal effectors was successfully generated, by crossing FL3#2 which carries a female-specific tTA overexpression cassette, with EF1#12 which carries a tTA-regulated LshidAla2 cassette. Females with one copy of the FL3#2 transgene are viable but up to 99.8% of homozygous females die at the pupal stage when raised on diet that lacks tetracycline. Additionally, the female lethality of FL3#2 was partially repressed by supplying tetracycline to the parental generation. With an additional LshidAla2 effector, the female lethality of DH6 is 100% dominant and cannot be repressed by maternal tetracycline. DH6 females die at the late-larval stage. Several fitness parameters important for mass rearing such as hatching rate, adult emergence and sex ratio were comparable to those of the wild type strain.ConclusionsCompared to the parental FL3#2 strain, the DH6 strain shows stronger female lethality and lethality occurs at an earlier stage of development. The combination of two tTA-dependent lethal effectors could improve strain stability under mass rearing and could reduce the risk of resistance in the field if fertile males are released. Our approach could be easily adapted for other pest species for an efficient, safe and sustainable genetic control program.

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Lucilia cuprina genome unlocks parasitic fly biology to underpin future interventions.

Publications

Lucilia cuprina genome unlocks parasitic fly biology to underpin future interventions.

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