Ontology highlight
ABSTRACT:
SUBMITTER: Binder BP
PROVIDER: S-EPMC4491736 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Binder Benjamin P BP Cornea Sinziana S Thompson Andrew R AR Moen Rebecca J RJ Thomas David D DD
Proceedings of the National Academy of Sciences of the United States of America 20150608 26
Using electron paramagnetic resonance (EPR) of a bifunctional spin label (BSL) bound stereospecifically to Dictyostelium myosin II, we determined with high resolution the orientation of individual structural elements in the catalytic domain while myosin is in complex with actin. BSL was attached to a pair of engineered cysteine side chains four residues apart on known α-helical segments, within a construct of the myosin catalytic domain that lacks other reactive cysteines. EPR spectra of BSL-myo ...[more]