TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?

ABSTRACT: TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance.

SUBMITTER: Sizova MV 

PROVIDER: S-EPMC4492686 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature