TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?
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ABSTRACT:
SUBMITTER: Sizova MV
PROVIDER: S-EPMC4492686 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?
Sizova Maria V MV Doerfert Sebastian N SN Gavrish Ekaterina E Epstein Slava S SS
Journal of microbiological methods 20150506
TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance. ...[more]
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