Ontology highlight
ABSTRACT:
SUBMITTER: Tong C
PROVIDER: S-EPMC4492704 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Tong Chengbi C Xiao Bin B Cheng Shasha S Li Wei W Liao Xiaoqing X Luo Shuhong S Hao Wenbo W
Monoclonal antibodies in immunodiagnosis and immunotherapy 20150601 3
Vacuolar proton pyrophosphatase (V-PPase), an electrogenic proton pump widely distributed in non-mammalian species, is one of the important targets for acidocalcisomes. In this study, a novel method of peptide-based antibody generation was performed to produce monoclonal antibodies (MAbs) against Toxoplasma gondii V-PPase. Three hybridomas were identified and confirmed by ELISA, Western blotting, and immunofluorescence. All of them can react with an 85 kDa band of T. gondii protein in purified a ...[more]