Unknown

Dataset Information

0

CENP-C is a blueprint for constitutive centromere-associated network assembly within human kinetochores.


ABSTRACT: Kinetochores are multisubunit complexes that assemble on centromeres to bind spindle microtubules and promote faithful chromosome segregation during cell division. A 16-subunit complex named the constitutive centromere-associated network (CCAN) creates the centromere-kinetochore interface. CENP-C, a CCAN subunit, is crucial for kinetochore assembly because it links centromeres with the microtubule-binding interface of kinetochores. The role of CENP-C in CCAN organization, on the other hand, had been incompletely understood. In this paper, we combined biochemical reconstitution and cellular investigations to unveil how CENP-C promotes kinetochore targeting of other CCAN subunits. The so-called PEST domain in the N-terminal half of CENP-C interacted directly with the four-subunit CCAN subcomplex CENP-HIKM. We identified crucial determinants of this interaction whose mutation prevented kinetochore localization of CENP-HIKM and of CENP-TW, another CCAN subcomplex. When considered together with previous observations, our data point to CENP-C as a blueprint for kinetochore assembly.

SUBMITTER: Klare K 

PROVIDER: S-EPMC4494010 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

CENP-C is a blueprint for constitutive centromere-associated network assembly within human kinetochores.

Klare Kerstin K   Weir John R JR   Basilico Federica F   Zimniak Tomasz T   Massimiliano Lucia L   Ludwigs Nina N   Herzog Franz F   Musacchio Andrea A  

The Journal of cell biology 20150629 1


Kinetochores are multisubunit complexes that assemble on centromeres to bind spindle microtubules and promote faithful chromosome segregation during cell division. A 16-subunit complex named the constitutive centromere-associated network (CCAN) creates the centromere-kinetochore interface. CENP-C, a CCAN subunit, is crucial for kinetochore assembly because it links centromeres with the microtubule-binding interface of kinetochores. The role of CENP-C in CCAN organization, on the other hand, had  ...[more]

Similar Datasets

| S-EPMC1283947 | biostudies-literature
| S-EPMC5223601 | biostudies-literature
| S-EPMC6003232 | biostudies-literature
| S-EPMC125570 | biostudies-literature
| S-EPMC6726658 | biostudies-literature
| S-EPMC3198334 | biostudies-literature
| S-EPMC2704923 | biostudies-literature
| S-EPMC5134894 | biostudies-literature
| S-EPMC2754938 | biostudies-literature
| S-EPMC1291240 | biostudies-literature