Stirred suspension bioreactors as a novel method to enrich germ cells from pre-pubertal pig testis.

ABSTRACT: To study spermatogonial stem cells the heterogeneous testicular cell population first needs to be enriched for undifferentiated spermatogonia, which contain the stem cell population. When working with non-rodent models, this step requires working with large numbers of cells. Available cell separation methods rely on differential properties of testicular cell types such as expression of specific cell surface proteins, size, density, or differential adhesion to substrates to separate germ cells from somatic cells. The objective of this study was to develop an approach that allowed germ cell enrichment while providing efficiency of handling large cell numbers. Here, we report the use of stirred suspension bioreactors (SSB) to exploit the adhesion properties of Sertoli cells to enrich cells ob

SUBMITTER: Dores C 

PROVIDER: S-EPMC4495971 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature