Unknown

Dataset Information

0

Chiral amine synthesis using ?-transaminases: an amine donor that displaces equilibria and enables high-throughput screening.


ABSTRACT: The widespread application of ?-transaminases as biocatalysts for chiral amine synthesis has been hampered by fundamental challenges, including unfavorable equilibrium positions and product inhibition. Herein, an efficient process that allows reactions to proceed in high conversion in the absence of by-product removal using only one equivalent of a diamine donor (ortho-xylylenediamine) is reported. This operationally simple method is compatible with the most widely used (R)- and (S)-selective ?-TAs and is particularly suitable for the conversion of substrates with unfavorable equilibrium positions (e.g., 1-indanone). Significantly, spontaneous polymerization of the isoindole by-product generates colored derivatives, providing a high-throughput screening platform to identify desired ?-TA activity.

SUBMITTER: Green AP 

PROVIDER: S-EPMC4497610 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Chiral amine synthesis using ω-transaminases: an amine donor that displaces equilibria and enables high-throughput screening.

Green Anthony P AP   Turner Nicholas J NJ   O'Reilly Elaine E  

Angewandte Chemie (International ed. in English) 20140819 40


The widespread application of ω-transaminases as biocatalysts for chiral amine synthesis has been hampered by fundamental challenges, including unfavorable equilibrium positions and product inhibition. Herein, an efficient process that allows reactions to proceed in high conversion in the absence of by-product removal using only one equivalent of a diamine donor (ortho-xylylenediamine) is reported. This operationally simple method is compatible with the most widely used (R)- and (S)-selective ω-  ...[more]

Similar Datasets

| S-EPMC9292530 | biostudies-literature
| S-EPMC3817511 | biostudies-literature
| S-EPMC3166231 | biostudies-literature
| S-EPMC5377235 | biostudies-literature
| S-EPMC3671589 | biostudies-other
| S-EPMC6874555 | biostudies-literature
| S-EPMC6332576 | biostudies-literature
| S-EPMC7570888 | biostudies-literature
| S-EPMC8150671 | biostudies-literature
| S-EPMC9926512 | biostudies-literature