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Fusion-protein-assisted protein crystallization.

ABSTRACT: Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the `heterologous fusion-protein approach', the fusion partner can provide additional surface area to promote crystal contact formation. In another approach, the `fusion of interacting proteins approach', protein assemblies can be stabilized by covalently linking the interacting partners. The linker connecting the proteins plays different roles in the two applications: in the first approach a rigid linker is required to reduce conformational heterogeneity; in the second, conversely, a flexible linker is required that allows the native interaction between the fused proteins. The two approaches can also be combined. The recent applications of fusion-protein technology in protein crystallization from the work of our own and other laboratories are briefly reviewed.

SUBMITTER: Kobe B

PROVIDER: S-EPMC4498707 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Fusion-protein-assisted protein crystallization.

Kobe Bostjan B Ve Thomas T Williams Simon J SJ

Acta crystallographica. Section F, Structural biology communications 20150627 Pt 7

Fusion proteins can be used directly in protein crystallization to assist crystallization in at least two different ways. In one approach, the `heterologous fusion-protein approach', the fusion partner can provide additional surface area to promote crystal contact formation. In another approach, the `fusion of interacting proteins approach', protein assemblies can be stabilized by covalently linking the interacting partners. The linker connecting the proteins plays different roles in the two app ...[more]

PMID: 26144231

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