Unknown

Dataset Information

0

Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys.


ABSTRACT: Sooty mangabeys (Cercocebus atys) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have characterized 121 functionally full-length classical (Ceat-A and Ceat-B) and non-classical (Ceat-F and Ceat-I) alleles and have also identified 22 Ceat-A/Ceat-B haplotype chromosomal combinations. We correlated these Ceat-A/Ceat-B haplotype combinations to recently described microsatellite haplotypes from the YNPRC colony. These newly identified alleles and haplotypes establish a resource for studying cellular immunity in sooty mangabeys and provide a framework for rapidly cataloging MHC class I sequences in an understudied, yet important, nonhuman primate species.

SUBMITTER: Heimbruch KE 

PROVIDER: S-EPMC4498956 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Novel MHC class I full-length allele and haplotype characterization in sooty mangabeys.

Heimbruch Katelyn E KE   Karl Julie A JA   Wiseman Roger W RW   Dudley Dawn M DM   Johnson Zach Z   Kaur Amitinder A   O'Connor David H DH  

Immunogenetics 20150526 8


Sooty mangabeys (Cercocebus atys) are natural SIV hosts and the presumed source of HIV-2 and SIVmac, which makes them a valuable model for HIV/SIV research. However, like other African primates, little is known about their major histocompatibility complex (MHC) genetics. In this study, we used Roche/454 and Illumina MiSeq deep sequencing in order to determine the MHC class I transcripts in a cohort of 165 sooty mangabeys from the Yerkes National Primate Research Center (YNPRC). We have character  ...[more]

Similar Datasets

| S-EPMC7153738 | biostudies-literature
| S-EPMC3910708 | biostudies-literature
| S-EPMC4536953 | biostudies-literature
| S-EPMC3156323 | biostudies-literature
| PRJEB46554 | ENA
| S-EPMC7089916 | biostudies-literature
| S-EPMC4378528 | biostudies-literature
| PRJEB23658 | ENA
| S-EPMC2813950 | biostudies-literature
| S-EPMC5717689 | biostudies-literature