Ontology highlight
ABSTRACT:
SUBMITTER: Pandey M
PROVIDER: S-EPMC4499146 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Pandey Manjula M Elshenawy Mohamed M MM Jergic Slobodan S Takahashi Masateru M Dixon Nicholas E NE Hamdan Samir M SM Patel Smita S SS
Nucleic acids research 20150524 12
The Escherichia coli replication terminator protein (Tus) binds to Ter sequences to block replication forks approaching from one direction. Here, we used single molecule and transient state kinetics to study responses of the heterologous phage T7 replisome to the Tus-Ter complex. The T7 replisome was arrested at the non-permissive end of Tus-Ter in a manner that is explained by a composite mousetrap and dynamic clamp model. An unpaired C(6) that forms a lock by binding into the cytosine binding ...[more]