Ontology highlight
ABSTRACT:
SUBMITTER: Devi SG
PROVIDER: S-EPMC4500551 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Devi Selvaraju Gayathri SG Fathima Anwar Aliya AA Radha Sudhakar S Arunraj Rex R Curtis Wayne R WR Ramya Mohandass M
PloS one 20150713 7
A rapid, cost effective method of metagenomic DNA extraction from soil is a useful tool for environmental microbiology. The present work describes an improved method of DNA extraction namely "powdered glass method" from diverse soils. The method involves the use of sterile glass powder for cell lysis followed by addition of 1% powdered activated charcoal (PAC) as purifying agent to remove humic substances. The method yielded substantial DNA (5.87 ± 0.04 μg/g of soil) with high purity (A260/280: ...[more]