Ontology highlight
ABSTRACT:
SUBMITTER: Gherghe CM
PROVIDER: S-EPMC4501255 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Gherghe Costin M CM Mortimer Stefanie A SA Krahn Joseph M JM Thompson Nancy L NL Weeks Kevin M KM
Journal of the American Chemical Society 20080618 28
RNA molecules undergo local conformational dynamics on timescales spanning picoseconds to minutes. Slower local motions have the greater potential to govern RNA folding, ligand recognition, and ribonucleoprotein assembly reactions but are difficult to detect in large RNAs with complex structures. RNA SHAPE chemistry employs acylation of the ribose 2'-hydroxyl position to measure local nucleotide flexibility in RNA and is well-characterized by a mechanism in which each nucleotide samples unreacti ...[more]