Ontology highlight
ABSTRACT:
SUBMITTER: Mueller S
PROVIDER: S-EPMC4501358 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Mueller Susanne S Wahlander Asa A Selevsek Nathalie N Otto Claudia C Ngwa Elsy Mankah EM Poljak Kristina K Frey Alexander D AD Aebi Markus M Gauss Robert R
Molecular biology of the cell 20150520 14
Protein degradation is essential for cellular homeostasis. We developed a sensitive approach to examining protein degradation rates in Saccharomyces cerevisiae by coupling a SILAC approach to selected reaction monitoring (SRM) mass spectrometry. Combined with genetic tools, this analysis made it possible to study the assembly of the oligosaccharyl transferase complex. The ER-associated degradation machinery compensated for disturbed homeostasis of complex components by degradation of subunits in ...[more]