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Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone.


ABSTRACT: The 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.

SUBMITTER: Park DJ 

PROVIDER: S-EPMC4503805 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone.

Park Daniel J DJ   Dudas Gytis G   Wohl Shirlee S   Goba Augustine A   Whitmer Shannon L M SL   Andersen Kristian G KG   Sealfon Rachel S RS   Ladner Jason T JT   Kugelman Jeffrey R JR   Matranga Christian B CB   Winnicki Sarah M SM   Qu James J   Gire Stephen K SK   Gladden-Young Adrianne A   Jalloh Simbirie S   Nosamiefan Dolo D   Yozwiak Nathan L NL   Moses Lina M LM   Jiang Pan-Pan PP   Lin Aaron E AE   Schaffner Stephen F SF   Bird Brian B   Towner Jonathan J   Mamoh Mambu M   Gbakie Michael M   Kanneh Lansana L   Kargbo David D   Massally James L B JL   Kamara Fatima K FK   Konuwa Edwin E   Sellu Josephine J   Jalloh Abdul A AA   Mustapha Ibrahim I   Foday Momoh M   Yillah Mohamed M   Erickson Bobbie R BR   Sealy Tara T   Blau Dianna D   Paddock Christopher C   Brault Aaron A   Amman Brian B   Basile Jane J   Bearden Scott S   Belser Jessica J   Bergeron Eric E   Campbell Shelley S   Chakrabarti Ayan A   Dodd Kimberly K   Flint Mike M   Gibbons Aridth A   Goodman Christin C   Klena John J   McMullan Laura L   Morgan Laura L   Russell Brandy B   Salzer Johanna J   Sanchez Angela A   Wang David D   Jungreis Irwin I   Tomkins-Tinch Christopher C   Kislyuk Andrey A   Lin Michael F MF   Chapman Sinead S   MacInnis Bronwyn B   Matthews Ashley A   Bochicchio James J   Hensley Lisa E LE   Kuhn Jens H JH   Nusbaum Chad C   Schieffelin John S JS   Birren Bruce W BW   Forget Marc M   Nichol Stuart T ST   Palacios Gustavo F GF   Ndiaye Daouda D   Happi Christian C   Gevao Sahr M SM   Vandi Mohamed A MA   Kargbo Brima B   Holmes Edward C EC   Bedford Trevor T   Gnirke Andreas A   Ströher Ute U   Rambaut Andrew A   Garry Robert F RF   Sabeti Pardis C PC  

Cell 20150601 7


The 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evide  ...[more]

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