Unknown

Dataset Information

0

Strategies for developing multi-epitope, subunit-based, chemically synthesized anti-malarial vaccines.


ABSTRACT: An anti-malarial vaccine against the extremely lethal Plasmodium falciparum is desperately needed. Peptides from this parasite's proteins involved in invasion and having high red blood cell-binding ability were identified; these conserved peptides were not immunogenic or protection-inducing when used for immunizing Aotus monkeys. Modifying some critical binding residues in these high-activiy binding peptides' (HABPs') attachment to red blood cells (RBC) allowed them to induce immunogenicity and protection against experimental challenge and acquire the ability to bind to specific HLA-DRp1* alleles. These modified HABPs adopted certain characteristic structural configurations as determined by circular dichroism (CD) and 1H nuclear magnetic resonance (NMR) associated with certain HLA-DRbeta1* haplotype binding activities and characteristics, such as a 2-angstroms-distance difference between amino acids fitting into HLA-DRp1 Pockets 1 to 9, residues participating in binding to HLA-DR pockets and residues making contact with the TCR, suggesting haplotype and allele-conscious TCR. This has been demonstrated in HLA-DR-like genotyped monkeys and provides the basis for designing high effective, subunit-based, multi-antigen, multi-stage, synthetic vaccines, for immediate human use, malaria being one of them.

SUBMITTER: Patarroyo ME 

PROVIDER: S-EPMC4506160 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Strategies for developing multi-epitope, subunit-based, chemically synthesized anti-malarial vaccines.

Patarroyo M E ME   Cifuentes G G   Bermúdez A A   Patarroyo M A MA  

Journal of cellular and molecular medicine 20081001 5B


An anti-malarial vaccine against the extremely lethal Plasmodium falciparum is desperately needed. Peptides from this parasite's proteins involved in invasion and having high red blood cell-binding ability were identified; these conserved peptides were not immunogenic or protection-inducing when used for immunizing Aotus monkeys. Modifying some critical binding residues in these high-activiy binding peptides' (HABPs') attachment to red blood cells (RBC) allowed them to induce immunogenicity and  ...[more]

Similar Datasets

| S-EPMC2846346 | biostudies-literature
| S-EPMC6518434 | biostudies-literature
| S-EPMC3849409 | biostudies-literature
| S-EPMC11362624 | biostudies-literature
| S-EPMC8597982 | biostudies-literature
| S-EPMC4810083 | biostudies-literature
| S-EPMC8437302 | biostudies-literature
| S-EPMC7824815 | biostudies-literature
| S-EPMC7527307 | biostudies-literature
| S-EPMC7441662 | biostudies-literature