Ontology highlight
ABSTRACT:
SUBMITTER: Erdmann I
PROVIDER: S-EPMC4507001 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Erdmann Ines I Marter Kathrin K Kobler Oliver O Niehues Sven S Abele Julia J Müller Anke A Bussmann Julia J Storkebaum Erik E Ziv Tamar T Thomas Ulrich U Dieterich Daniela C DC
Nature communications 20150703
The specification and adaptability of cells rely on changes in protein composition. Nonetheless, uncovering proteome dynamics with cell-type-specific resolution remains challenging. Here we introduce a strategy for cell-specific analysis of newly synthesized proteomes by combining targeted expression of a mutated methionyl-tRNA synthetase (MetRS) with bioorthogonal or fluorescent non-canonical amino-acid-tagging techniques (BONCAT or FUNCAT). Substituting leucine by glycine within the MetRS-bind ...[more]