Ontology highlight
ABSTRACT:
SUBMITTER: Normanno D
PROVIDER: S-EPMC4507003 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Normanno Davide D Boudarène Lydia L Dugast-Darzacq Claire C Chen Jiji J Richter Christian C Proux Florence F Bénichou Olivier O Voituriez Raphaël R Darzacq Xavier X Dahan Maxime M
Nature communications 20150707
Many cellular functions rely on DNA-binding proteins finding and associating to specific sites in the genome. Yet the mechanisms underlying the target search remain poorly understood, especially in the case of the highly organized mammalian cell nucleus. Using as a model Tet repressors (TetRs) searching for a multi-array locus, we quantitatively analyse the search process in human cells with single-molecule tracking and single-cell protein-DNA association measurements. We find that TetRs explore ...[more]