Ontology highlight
ABSTRACT:
SUBMITTER: Chen TY
PROVIDER: S-EPMC4507017 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Chen Tai-Yen TY Santiago Ace George AG Jung Won W Krzemiński Łukasz Ł Yang Feng F Martell Danya J DJ Helmann John D JD Chen Peng P
Nature communications 20150706
Binding and unbinding of transcription regulators at operator sites constitute a primary mechanism for gene regulation. While many cellular factors are known to regulate their binding, little is known on how cells can modulate their unbinding for regulation. Using nanometer-precision single-molecule tracking, we study the unbinding kinetics from DNA of two metal-sensing transcription regulators in living Escherichia coli cells. We find that they show unusual concentration-dependent unbinding kin ...[more]