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Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing.


ABSTRACT: Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

SUBMITTER: Bjorn-Mortensen K 

PROVIDER: S-EPMC4508433 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to Whole-Genome Sequencing.

Bjorn-Mortensen K K   Zallet J J   Lillebaek T T   Andersen A B AB   Niemann S S   Rasmussen E M EM   Kohl T A TA  

Journal of clinical microbiology 20150527 8


Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks. ...[more]

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