Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death.
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ABSTRACT:
SUBMITTER: Dixon SJ
PROVIDER: S-EPMC4509420 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death.
Dixon Scott J SJ Winter Georg E GE Musavi Leila S LS Lee Eric D ED Snijder Berend B Rebsamen Manuele M Superti-Furga Giulio G Stockwell Brent R BR
ACS chemical biology 20150515 7
Little is known about the regulation of nonapoptotic cell death. Using massive insertional mutagenesis of haploid KBM7 cells we identified nine genes involved in small-molecule-induced nonapoptotic cell death, including mediators of fatty acid metabolism (ACSL4) and lipid remodeling (LPCAT3) in ferroptosis. One novel compound, CIL56, triggered cell death dependent upon the rate-limiting de novo lipid synthetic enzyme ACC1. These results provide insight into the genetic regulation of cell death a ...[more]
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