Ontology highlight
ABSTRACT:
SUBMITTER: Wyvekens N
PROVIDER: S-EPMC4509490 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Wyvekens Nicolas N Topkar Ved V VV Khayter Cyd C Joung J Keith JK Tsai Shengdar Q SQ
Human gene therapy 20150701 7
Monomeric clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated 9 (Cas9) nucleases have been widely adopted for simple and robust targeted genome editing but also have the potential to induce high-frequency off-target mutations. In principle, two orthogonal strategies for reducing off-target cleavage, truncated guide RNAs (tru-gRNAs) and dimerization-dependent RNA-guided FokI-dCas9 nucleases (RFNs), could be combined as tru-RFNs to further improve genome editing sp ...[more]