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On the Effect of Sodium Chloride and Sodium Sulfate on Cold Denaturation.

ABSTRACT: Both sodium chloride and sodium sulfate are able to stabilize yeast frataxin, causing an overall increase of its thermodynamic stability curve, with a decrease in the cold denaturation temperature and an increase in the hot denaturation one. The influence of low concentrations of these two salts on yeast frataxin stability can be assessed by the application of a theoretical model based on scaled particle theory. First developed to figure out the mechanism underlying cold denaturation in water, this model is able to predict the stabilization of globular proteins provided by these two salts. The densities of the salt solutions and their temperature dependence play a fundamental role.

SUBMITTER: Pica A

PROVIDER: S-EPMC4511003 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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On the Effect of Sodium Chloride and Sodium Sulfate on Cold Denaturation.

Pica Andrea A Graziano Giuseppe G

PloS one 20150721 7

Both sodium chloride and sodium sulfate are able to stabilize yeast frataxin, causing an overall increase of its thermodynamic stability curve, with a decrease in the cold denaturation temperature and an increase in the hot denaturation one. The influence of low concentrations of these two salts on yeast frataxin stability can be assessed by the application of a theoretical model based on scaled particle theory. First developed to figure out the mechanism underlying cold denaturation in water, t ...[more]

PMID: 26197394

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