Ontology highlight
ABSTRACT:
SUBMITTER: Fan X
PROVIDER: S-EPMC4511241 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Fan Xiaoying X Zhang Xiannian X Wu Xinglong X Guo Hongshan H Hu Yuqiong Y Tang Fuchou F Huang Yanyi Y
Genome biology 20150723
Circular RNAs (circRNAs) are a new class of non-polyadenylated non-coding RNAs that may play important roles in many biological processes. Here we develop a single-cell universal poly(A)-independent RNA sequencing (SUPeR-seq) method to sequence both polyadenylated and non-polyadenylated RNAs from individual cells. This method exhibits robust sensitivity, precision and accuracy. We discover 2891 circRNAs and 913 novel linear transcripts in mouse preimplantation embryos and further analyze the abu ...[more]